Spectroscopy with “Integrating Cavity” enhances performance to include:
3 Stories of Clarity Owners:
Douglas A. Campbell, Ph.D.
Professor & Canada Chair in Phytoplankton Ecophysiology
Mount Allison University
New Brunswick, Canada
Dr. Campbell’s story:
Doug Campbell did not know OLIS and we did not know him when he learned about the CLARiTY. We had made a fiscal contribution to a plant physiology meeting he attended and he noticed our flyer introducing the CLARiTY. Immediately, he stopped the purchase of a mass-produced UV/Vis with integrating sphere and purchased an OLIS CLARiTY built around the modernized Cary 17 UV/Vis/NIR spectrophotometer. Today, this instrument is bedecked with a sign reading “Spectrophotometer of the Gods.” When it came time to purchase another spectrometer, Doug worked with us to design a “pump-probe” model which will be delivered in early 2015. This system is physically tiny relative to the CLARiTY 17 and has the unique ability to ‘pump’ the sample with microsecond precision actinic illumination from up to 7 LEDs and then ‘probe’ the suspension of living cells with UV/Vis spectra.
Congratulations and continuing success to the purchaser of CLARiTY #5 (the model 17) and CLARiTY #23 (the model VF Pump Probe), who identifies his work as “We study the ecophysiology of phytoplankton, with particular emphases on Photosystem II and the Photosystem II inactivation/regeneration cycle, resource allocations and analyses of the costs of growth and acclimation to environmental variation, and new, integrative approaches to quantify and analyze these processes.”
Pictured below is the CLARiTY 17 in Doug’s lab adorned with a homemade “Spectrophotometer of the Gods” sign.
At right is data sent to OLIS by Dr. Campbell’s lab in April, 2014.
CLARiTY Publications:
Rising CO2 Interacts with Growth Light and Growth Rate to Alter Photosystem II Photoinactivation of the Coastal Diatom Thalassiosira pseudonana
LoS ONE 8(1): e55562.
The RUBISCO to Photosystem II Ratio Limits the Maximum Photosynthetic Rate in Picocyanobacteria
Life 2015, 5, 403-417.
Eugene Pinkhassik, Ph.D.
Associate Professor & NSF CAREER Award Recipient
UCONN
Connecticut, USA
Dr. Pinkhassik’s story:
Eugene Pinkhassik had a few years under his belt as an OLIS client, owning an OLIS modernized SLM 8000 spectrofluorimeter, when he learned about our CLARiTY. The timing was perfect: he was moving from the University of Memphis to St. Louis University. So, he sent a post-doc and student here with samples. The results acquired during this day won him and his people over. Within a few months, they impressed the editors of Analytical Chemistry enough to land the coveted cover for that month. Fast forward to his recent move to UConn. His first CLARiTY remains in St. Louis and his second CLARiTY has been purchased for delivery in early 2015. When complimenting him on the number of publications his group has already made, his splendid reply was that “You ain’t seen nothing yet!”
Congratulations and continuing success to the purchaser of CLARiTY #4 (the model 620) and CLARiTY #24 (the model 1000), who identifies his research as “Making nanomaterials and nanodevices with new and superior properties to address current problems in energy-related technologies, medical imaging and treatment, and environmental sensing.”
CLARiTY Publications:
Controlled Permeability in Porous Polymer Nanocapsules Enabling Size-and Charge-Selective SERS Nanoprobes
ACS Appl. Mater. Interfaces, 2016, 8 (30), pp 19755–19763.
Controlling the Encapsulation of Charged Molecules in Vesicle-Templated Nanocontainers through Electrostatic Interactions with the Bilayer Scaffold
Langmuir, 2017, 33 (31), pp 7732–7740.
Design of Fluorescent Nanocapsules as Ratiometric Nanothermometers
Chem. Eur. J. 2014, 20: 10292–10297.
Dye-Loaded Porous Nanocapsules Immobilized in a Permeable Polyvinyl Alcohol Matrix: A Versatile Optical Sensor Platform
Anal. Chem. 2012, 84, 2695−2701
Catalytic ship-in-a-bottle assembly within hollow porous nanocapsules
New J. Chem., 2014,38, 481-485
Selectively Initiated Ship-In-A-Bottle Assembly of Yolk–Shell Nanostructures
Chem. Mater., 2014, 26 (2), 1126–1132
Synergistic Co-Entrapment and Triggered Release in Hollow Nanocapsules with Uniform Nanopores
J. Am. Chem. Soc., 2011, 133 (49), 19656–19659
Blood triggered rapid release porous nanocapsules
RSC Advances, 2013, 3, 5547
Robert K. Poole, Ph.D.
Professor & West Riding Chair in Microbiology
University of Sheffield
Sheffield, UK
Dr. Poole’s story:
Figure from Dr. Poole’s Microbiology paper comparing spectra of Salmonella in situ with a Standard Dual Beam UV/Vis (A) and the CLARiTY (B).
CLARiTY Publications:
Interaction of the carbon monoxide-releasing molecule Ru(CO)3Cl(glycinate) (CORM-3) with Salmonella enterica serovar Typhimurium: in situ measurements of CO binding by integrating cavity dual beam spectrophotometry
Microbiology, 2014, Published online ahead of print, 8.1.14
Introducing [Mn(CO)3(tpa-κ3N)]+ as a novel photoactivatable CO-releasing molecule with well-defined iCORM intermediates – synthesis, spectroscopy, and antibacterial activity
Dalton Trans., 2014,43, 9986-9997
Dr. Poole’s story:
Robert Poole had known the reputation of Richard DeSa for many years, because of a shared acquaintance with Quentin Gibson and colleagues who had purchased OLIS spectrophotometers, but it was when he was in the market for a stopped-flow spectrometer himself that their conversation started. Robert quickly saw the superior performance of the OLIS RSM 1000 rapid-scanning spectrophotometer over diode array detection for stopped-flow studies. And, when he learned that DeSa had developed a second application for this RSM 1000 – the scatter immune CLARiTY – the decision to choose OLIS was made. Poole became only the second scientist in the world to purchase a CLARiTY and the first in the UK. After a year of fruitful kinetic studies, his group turned their attention to the CLARiTY, quickly confirming firsthand that the results were “very different and very superior” to those from the Johnson Foundation dual wavelength spectrometer they have long relied upon for turbid samples. In January 2014, Poole reported “So, three different bacteria, 3 Olis papers!” Unknown to him, in 2010, John S. Olson (like DeSa, a former member of Gibson’s laboratory) anticipated “If Robert [Poole] and his crew can do whole bacteria experiments successfully with in vivo flavoHb and other globins, the system will sell itself.”
Congratulations and continuing success to the purchaser of CLARiTY #2 (the model 1000), who describes his work as “to understand the structure,function, molecular genetics and biosynthesis of microbial respiratory proteins, especially the terminal oxidases and haemoglobins of bacteria. In addition, we work on the bioinorganic chemistry of metal function and toxicity in microorganisms, and the influence of oxygen levels in bacterial biofilms.”
Robert K. Poole, Ph.D.
Professor & West Riding Chair in Microbiology
University of Sheffield
Sheffield, UK
“No centrifuging was necessary [while using the CLARiTY] to collect data you will like. It is very different. And very superior to results from the Johnson Foundation [dual wavelength] instrument’s spectra. We will be publishing soon.”
Prof Robert Poole, May 2013